Particle Explorer

Sample Preparation

Filtration

The gold-coated filters (filtr.AID membranes with 0.8, 3, 5, and 10 µm pore sizes) are perfect substrates for ensuring both robust counting and Raman signals without substrate interference.

Dry dispersion

Standardized and computer-controlled powder dispersion pushes powders into vacuum. The particles fall slowly and are perfectly distributed on any kind of substrate.

Wet dispersion

Specifically designed to obtain perfect powerful low-noise Raman signals from aggregates and particulates in bio therapeutics.

 

 

To solve the problems, we have a novel design of a wet cell optimized for Raman spectroscopy in protein based formulations. Sampling with the wet cell preserves not only the shapes of semi-solid and even liquid particles, it also bridges from Flow Imaging (shadow counting) to scientific proof. Examples of the measurement of inherent particles, mainly protein aggregates in a highly concentrated antibody formulation demonstrate the low spectroscopic background of this wet-dispersion.AID design. With the integrated image-directed spectroscopy, we can identify silicone droplets (intrinsic particles) that give great Raman signals down to 1 µm diameter and fatty acids resulting from the hydrolysis of polysorbate. Any user will also be able to analyze larger visible particles > 80 µm easily with this setup.

protein aggregates in the wet dispersion unit for in situ Raman spectroscopy of sub-visible particulate matter in biopharmaceutical formulations